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L-asparaginase II Production by Escherichia coli

Description: Growth of Escherichia coli A-l under aerobic conditions in an enriched medium with a total amount of 0.2 per cent glucose was biphasic and asparaginase II activity was detected after depletion of ammonia from the growth medium in the second phase of growth. Glucose was exhausted two hours before ammonia and three hours before asparaginase II activity was detected. The concentration of 3',5'-cyclic adenosine monophosphate was found to fluctuate when the dissolved oxygen in the medium reached a l… more
Date: May 1985
Creator: Johnson, Terrance L. (Terrance Lewyne), 1950-
Partner: UNT Libraries
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Comparative Biochemistry and Evolution of Aspartate Transcarbamoylase from Diverse Bacteria

Description: Aspartate transcarbamoylase (ATCase) catalyzes the first committed step in pyrimidine biosynthesis. Bacterial ATCases are divided into three classes, A, B and C. Class A ATCases are largest at 450-500, are. dodecamers and represented by Pseudomonas ATCase. The overlapping pyrBC' genes encode the Pseudomonases ATCase, which is active only as a 480 kDa dodecamer and requires an inactive pyrC'-encoded DHOase for ATCase activity. ATCase has been studied in two non-pathogenic members of Mycobacteriu… more
Date: May 1999
Creator: Hooshdaran, Massoumeh Ziba
Partner: UNT Libraries
open access

A Study of the Water-Soluble Antigens from Virulent and Attenuated Biotypes of Brucella abortus

Description: Through chemical analysis and ion exchange chromatography of watersoluble antigens, this investigation supports the view that the majority of differences between the biotypes are quantitative. It was also found that strains demonstrate distinct, qualitative differences when compared to the attenuated strain 19 by immunodiffusion and thin-layer polyacrylamide gel, isoelectric focusing. These differences include the presence of antigens on virulent strains that are absent on strain 19. In additio… more
Date: May 1977
Creator: Brodeur, Richard D.
Partner: UNT Libraries
open access

Characterization of the Pigment-Protein and Pigment-ester of Xanthomonas Campestris Pv. Juglandis

Description: The objectives of this project were to develop a high performance liquid chromatographic method for separating the pigment esters mixture, to determine the locations of the pigment moiety in the isolated esters using pholosiphases, and to characterize the pigment-protein complex and determine its distribution in other bacteria. Saponification of the two pigment esters 1 and 2 with aqueous KOH yielded two free pigments on TLC plates developed by two solvent systems. The fasters moving of these t… more
Date: May 1987
Creator: Lawani, Leonard Olu
Partner: UNT Libraries
open access

Characterization of the Pigment-Protein Complex in Corynebacterium Poinsettiae

Description: The purpose of this study was to completely characterize the protein moiety in the caroteno complex in C. poinsettae, determine if the distribution and level of protein in the pigment-protein complex in membranes of the wild type and in a colorless mutant could account for the differences in the stability of the membrane, and to determine if this protein is common to other pigmented and non-pigmented organisms. Also, electron microscopy of cell membranes of C. poinsettiae which had been exposed… more
Date: May 1986
Creator: Ebadati, Nasrollah D.
Partner: UNT Libraries
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