A Study of the Water-Soluble Antigens from Virulent and Attenuated Biotypes of Brucella abortus

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Through chemical analysis and ion exchange chromatography of watersoluble antigens, this investigation supports the view that the majority of differences between the biotypes are quantitative. It was also found that strains demonstrate distinct, qualitative differences when compared to the attenuated strain 19 by immunodiffusion and thin-layer polyacrylamide gel, isoelectric focusing. These differences include the presence of antigens on virulent strains that are absent on strain 19. In addition, one antigen absent on strain 19, was found common to each virulent biotype. Finally, the results from immunodiffusion experiments, employing adsorbed and non-adsorbed immune globulins, indicate that at least some water-soluble antigens … continued below

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viii, 62 leaves: ill.

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Brodeur, Richard D. May 1977.

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  • Brodeur, Richard D.

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Through chemical analysis and ion exchange chromatography of watersoluble antigens, this investigation supports the view that the majority of differences between the biotypes are quantitative. It was also found that strains demonstrate distinct, qualitative differences when compared to the attenuated strain 19 by immunodiffusion and thin-layer polyacrylamide gel, isoelectric focusing. These differences include the presence of antigens on virulent strains that are absent on strain 19. In addition, one antigen absent on strain 19, was found common to each virulent biotype. Finally, the results from immunodiffusion experiments, employing adsorbed and non-adsorbed immune globulins, indicate that at least some water-soluble antigens are exposed on the cell surface and that their distribution among the biotypes varies.

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viii, 62 leaves: ill.

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  • May 1977

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Brodeur, Richard D. A Study of the Water-Soluble Antigens from Virulent and Attenuated Biotypes of Brucella abortus, thesis, May 1977; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc504302/: accessed May 26, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .

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