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Article discussing auditory cortex neurons and primary culture and ion channel activity in rat.
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7 p.
Notes
Abstract: We have developed a primary dissociated cell culture of the fetal (E17) and post-natal (P0-P10) rat auditory cortex. Pyramidal and non-pyramidal cells had a mean cross-sectional diameter of 12.73 ± 1.80 μm (mean ± S.D., n=25) and 17.58 ± 1.67 pm (mean ± S.D., n=10), respectively, measured at 6 days in culture. These cells were viable for as long as 18-21 days. They expressed voltage-gated sodium and potassium channel currents as early as one day in culture, and at various phases in cell culture. Sodium current, activated at membrane potentials more positive than -60 mV, displayed fast activation and inactivation kinetics. Fifty percent inactivation of sodium channels occurred at a pre-pulse potential of -63 mV. Delayed rectifier potassium channels were activated at potentials positive to -40 mV. Large hyperpolarizing constant current pulses elicited anode break action potentials, and large depolarizing constant current pulses exhibited rectification indicative of the delayed rectifying potassium channel activity.
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Moore, Ernest J.; Hall, Deanne B. & Narahashi, Toshio.Auditory Cortex Neurons: Primary Culture and Ion Channel Activity in Rat,
article,
February 28, 1995;
(https://digital.library.unt.edu/ark:/67531/metadc287992/:
accessed May 28, 2024),
University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu;
crediting UNT College of Arts and Sciences.
