Construction of a Physical Map of Moraxella (Branhamella) catarrhalis Strain ATCC25238

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In order to gain a better understanding of this microorganismand its role in human pathogenesis, a physical map of Moraxella catarrhalis type strain ATCC25238 was constructed using pulsed field gel electrophoresis (PFGE) in combination with Southern hybridization techniques. Restriction endonucleases Not I, Rsr II, and Sma I were used to digest the chromosomal DNA. An overlapping circular map was generated by cross-hybridization of isolated radiolabeled fragments of Moraxella catarrhalis genomic DNA to dried PFGE gels. The number and location of the 16S and 23S ribosomal RNA genes were determined by digestion with l-Ceul enzyme and by Southern hybridization. Virulence-associated genes, … continued below

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vi, 49 leaves : ill.

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Nguyen, Kim Thuy May 1999.

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This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided by the UNT Libraries to the UNT Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 332 times. More information about this thesis can be viewed below.

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  • Nguyen, Kim Thuy

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In order to gain a better understanding of this microorganismand its role in human pathogenesis, a physical map of Moraxella catarrhalis type strain ATCC25238 was constructed using pulsed field gel electrophoresis (PFGE) in combination with Southern hybridization techniques. Restriction endonucleases Not I, Rsr II, and Sma I were used to digest the chromosomal DNA. An overlapping circular map was generated by cross-hybridization of isolated radiolabeled fragments of Moraxella catarrhalis genomic DNA to dried PFGE gels. The number and location of the 16S and 23S ribosomal RNA genes were determined by digestion with l-Ceul enzyme and by Southern hybridization.
Virulence-associated genes, the gene for β-lactamase, and housekeeping genes were also placed onto the physical map.

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vi, 49 leaves : ill.

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  • May 1999

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  • March 26, 2014, 9:30 a.m.

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  • April 6, 2020, 7:41 p.m.

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Thumbnail image of item number 1 in: 'Construction of a Physical Map of Moraxella (Branhamella) catarrhalis Strain ATCC25238'.
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Nguyen, Kim Thuy. Construction of a Physical Map of Moraxella (Branhamella) catarrhalis Strain ATCC25238, thesis, May 1999; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc279104/: accessed May 26, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .

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