An Alcohol Drip Apparatus for the Sliding Microtome
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Description
In wet sectioning of celloidin embedded tissue it is necessary to maintain a layer of alcohol on the knife and on the surface of the block. The alcohol acts as a lubricant and also prevents drying of the block. The application of alcohol may be done by hand or mechanically. To apply the alcohol by hand while operating the microtome slows down the cutting operation, unless the operator is ambidextrous. In addition, stopping and starting the knife to apply alcohol results in an extensive wasting of tissue due to half-sections and sections of uneven thickness. Unless the knife can be …
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Added Title:
Brookhaven National Laboratory Report BNL-1643
Description
In wet sectioning of celloidin embedded tissue it is necessary to maintain a layer of alcohol on the knife and on the surface of the block. The alcohol acts as a lubricant and also prevents drying of the block. The application of alcohol may be done by hand or mechanically. To apply the alcohol by hand while operating the microtome slows down the cutting operation, unless the operator is ambidextrous. In addition, stopping and starting the knife to apply alcohol results in an extensive wasting of tissue due to half-sections and sections of uneven thickness. Unless the knife can be kept moving smoothly at a constant rate the result will be sections of non-uniform thickness regardless of the setting on the microtome.
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