A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield
Description:
This article develops a method, using a single DNA construct that uses CRISPR–Cas9 gene editing to knock-out expression of an endogenous gene of lignin monomer biosynthesis while at the same time expressing a modified version of the gene’s open reading frame that escapes cutting by the Cas9 system and complements the introduced mutation in a tissue-specific manner. This method is applicable to any plant species in which transformation and gene editing are feasible and validated vessel-specific …
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Date:
September 3, 2021
Creator:
Yu, Hasi; Liu, Chang & Dixon, R. A.
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Partner:
UNT College of Science