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RNaseH-mediated simultaneous piggyback knockdown of multiple genes in adult zebrafish

Description: This article tests if it is possible to piggyback more than one antisense deoxyoligonucleotide (dO) with one vivo morpholino (VMO). The authors previously developed a piggyback knockdown method that was used to knockdown genes in adult zebrafish. In this article, the authors develop a method to knockdown three genes at one time, and by increasing the concentration of VMO by twofold, could knockdown six genes simultaneously. These multiple gene knockdowns will not only increase the efficiency of… more
Date: November 19, 2020
Creator: Raman, Revathi; Ryon, Mia & Jagadeeswaran, Pudur
Item Type: Refine your search to only Article
Partner: UNT College of Science
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