Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy
Description:
The purpose of this research was to expand the chemiluminescence microscopy applications in live bacterial/mammalian cell imaging and to improve the detection sensitivity for ATP leaking or release events. We first demonstrated that chemiluminescence (CL) imaging can be used to interrogate single bacterial cells. While using a luminometer allows detecting ATP from cell lysate extracted from at least 10 bacterial cells, all previous cell CL detection never reached this sensitivity of single bact…
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Date:
December 18, 2008
Creator:
Zhang, Yun
Partner:
UNT Libraries Government Documents Department