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Supersensitive in Situ Hybridization by Tyramide Signal Amplification and Nanogold Silver Staining: The Contribution of Autometallography and Catalyzed Reporter Deposition to the Rejuvenation of In Situ Hybridization

Description: It is peculiar that in situ hybridization (ISH), a technique with many similarities to immunohistochemistry (IHC), has not enjoyed the phenomenal growth in both basic research and clinical applications as has its sister technique IHC. Since the late 1970s, when immunoperoxidase techniques began to be applied to routine diagnostic material and to numerous research applications, there has been a natural evolution of the IHC procedure. Namely, only a few primary antibodies were available commercia… more
Date: April 17, 2002
Creator: Tubbs, Raymond R.; Pettay, James; Grogan, Thomas; Powell, R. D.; Cheung, Annie L. M.; Hainfeld, James et al.
Partner: UNT Libraries Government Documents Department

Oral History Interview with Frank Curre, Jr., April 19, 2002

Description: Interview with Navy veteran Frank Curre Jr. The interview includes Curre's personal experiences about joining the Navy, boot camp, being aboard the battleship USS Tennessee during the Japanese attack at Pearl Harbor, and his subsequent experiences in the Pacific Theater during World War II. Additionally, Curre talks about pre-war shipboard life and training exercises, his activities during the attack on Pearl Harbor, aftermath of the attack, his transfer to the yard minesweeper YMS-102 at Breme… more
Access: Restricted to UNT Community Members. Login required if off-campus.
Date: April 19, 2002
Creator: Marcello, Ronald E. & Curre, Frank, Jr.
Partner: UNT Oral History Program
open access

Silver and Gold Based Autometallography of Nanogold.

Description: For many applications, silver salt-based autometallography (often also called silver enhancement or silver development) is required to visualize colloidal gold (1-5 nm in diameter) or the small 1.4 nm Nanogold{reg_sign} particles (Nanoprobes, Yaphank, NY, USA). Although even Nanogold may be seen directly by scanning-transmission electron microscopy (STEM), by transmission EM (TEM; in thin sections without stain or ice-embedded cryo-EM samples), energy filtered TEM, and scanning EM (SEM), silver… more
Date: April 17, 2002
Creator: Hainfeld, J. F. & Powell, R. D.
Partner: UNT Libraries Government Documents Department
open access

Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations.

Description: Nanogold{reg_sign}, a gold cluster with a core of gold atoms 1.4 nm in diameter, has proven to be a superior probe label for electron microscopy (EM), giving both higher labeling density and improved access to previously hindered or restricted antigens. It may be visualized by autometallography (AMG) for use in light microscopy (LM): silver-and gold-amplified Nanogold detection has proven to be one of the most sensitive methods available for the detection of low copy number targets such as vira… more
Date: April 17, 2002
Creator: Powell, R. D. & Hainfeld, J. F.
Partner: UNT Libraries Government Documents Department
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