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open access

Carbachol- and ACPD-Induced Phosphoinositide Responses in the Developing Rat Neocortex

Description: Signal transduction via the phosphoinositide (PI) second messenger system has key roles in the development and plasticity of the neocortex. The present study localized PI responses to individual cortical layers in slices of developing rat somatosensory cortex. The acetylcholine agonist carbachol and the glutamate agonist trans-1-amino-1,3-cyclopentanedicarboxylic acid (ACPD) were used to stimulate PI turnover. The PI responses were compared to the distribution of the corresponding PI-linked rec… more
Date: August 2000
Creator: Hartgraves, Morri D.
Partner: UNT Libraries
open access

Degradation of Humic Substances by Aquatic Bacteria

Description: A variety of aquatic bacteria were isolated and tested for their ability to degrade humic substances and their aromatic residues/monomers which serve as precursors of the trihalomethanes (THMs) found in chlorinated drinking waters. The majority of them were Gram-negative, oxidative types dominated by pseudomonads. Most of the 146 isolates were found to utilize as their sole source of carbon several or more of ten aromatic compounds known to be products of degradation of humus and also to be pre… more
Date: August 1985
Creator: Baiu, Saleh Hamed Salem
Partner: UNT Libraries
open access

Requirements for Cell-Free Cyanide Oxidation by Pseudomonas Fluorescens NCIMB 11764

Description: The involvement of cyanide oxygenase in the metabolism of pyruvate and a-ketoglutarate-cyanohydrin was investigated and shown to occur indirectly by the consumption of free cyanide arising from the cyanohydrins via chemical dissociation. Thus, free cyanide remains the substrate, for which the enzyme displays a remarkably high affinity (Kmapp,4 mM). A model for cyanide utilization is therefore envisioned in which the substrate is initially detoxified by complexation to an appropriate lig… more
Date: August 2000
Creator: Parab, Preeti
Partner: UNT Libraries

BioInformatics, Phylogenetics, and Aspartate Transcarbamoylase

Description: In this research, the necessity of understanding and using bioinformatics is demonstrated using the enzyme aspartate transcarbamoylase (ATCase) as the model enzyme. The first portion of this research focuses on the use of bioinformatics. A partial sequence of the pyrB gene found in Enterococcus faecalis was submitted to GenBank and was analyzed against the contiguous sequence from its own genome project. A BLAST (Basic Local Alignment Search Tool; Atschul, et al., 1990) was performed in or… more
Access: Restricted to UNT Community Members. Login required if off-campus.
Date: August 2000
Creator: Cooke, Patrick Alan
Partner: UNT Libraries

Adherence and Haemagglutination of Moraxella Catarrhalis.

Description: M. catarrhalis is a gram-negative diplococci frequently associated with infections of the upper respiratory tract. During the past decade, some preliminary studies have attempted to elucidate mechanisms of adherence and haemagglutination of M. catarrhalis. These studies have reported, in many cases, inconsistent results. There are two purposes of this research. First, identify mechanisms that may potentially be associated with the adherence and haemagglutination of M. catarrhalis. Second, sug… more
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Date: August 2000
Creator: Kosterman, Edward, III
Partner: UNT Libraries
open access

Aquatic Heterotrophic Bacteria Active in the Biotransformation of Anthracene and Pentachlorophenol

Description: Dominant genera of bacteria were isolated from three river waters during anthracene and pentachlorophenol biotransformation studies. The genera Pseudomonas, Acinetobacter, Micrococcus, Chromobacterium, Alcaligenes, Azomonos, Bacillus, and Flavobacterium were capable of biotransforming one or both of these compounds. These isolates were subjected to further biotransformation tests, including river water and a basal salt medium with and without additional glucose. The results of these experiments… more
Date: August 1985
Creator: Entezami, Azam A. (Azam Alsadat)
Partner: UNT Libraries
open access

The Detection of Poliovirus in Denton Sewage by Immunofluorescence and Immunodiffusion Techniques

Description: Several final sewage effluents from the Denton Disposal Plant were demonstrated to contain Poliovirus types II and III. Pleated encapsulated filters at pH3.5 enhanced the recovery of the Poliovirus at a higher tier in comparison with nitrocellulose filter (Millipore) and glass fiber filter of pore size 0.45u. This thesis explores problems that face us today in our quest to eliminate viral pathogens from the natural and waste water needed for human, domestic, and industrial consumption. Prelimin… more
Date: August 1975
Creator: Olaiya, Felix Ayodele
Partner: UNT Libraries
open access

Studies of the Membrane and DNA Gyrase Inhibiting Antibiotics on Pigment Synthesis in Corynebacterium Poinsettiae

Description: The purpose of this study was (1) to determine whether a correlation exists among the protein profiles, extracted from cell membranes of mutants belonging to five pigment cluster groups, (2) to locate the protein moiety and cartenoprotein complex in the membranes of wild type and colorless mutant (designated W-19) of C. poinsettae and to show whether there are any structural differences between cell membranes of the wild type and a colorless mutant, (3) to determine the effect of six antibioti… more
Date: August 1988
Creator: Tabarya, Daniel
Partner: UNT Libraries
open access

Immune Response of the Rat to Outer Membrane Proteins of Legionella Pneumophila

Description: Outer membrane proteins (OMPs) were recovered from eleven strains (eight serogroups) of Legionella pneumophila by sequential treatment with Tris buffer (pH 8), citrate buffer(pH 2.75) and Tris buffer (pH 8). Transmission electron microscopy revealed clearly the separation of the outer membrane from the bacteria. The development of delayed hypersensitivity was also noted by measuring the area of arythema and induration produced by intradermal injections of the MPSs from Chicago 8 strain. The adj… more
Date: August 1985
Creator: Ahanotu, Ejemihu Ndu
Partner: UNT Libraries
open access

Discovery of a Thermophilic Nitrogen Fixing Bacterium

Description: The thermophilic bacterium designated NT-7 was shown to reduce acetylene to ethylene at 35 C. It was found that the organism does not reduce acetylene when it is grown in Burk's medium with 0.3 per cent (w/v) NH4 N0 3 . Reduction of acetylene at 55 C could not be demonstrated due to insolubility of acetylene in Burk's medium at this temperature. It was shown that the bacterium NT-7 can not grow in the absence of atmospheric nitrogen at 55 C when combined nitrogen is not supplied with the nutrie… more
Date: August 1977
Creator: Tabarya, Daniel
Partner: UNT Libraries
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