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Mechanism of Excretion of a Bacterial Proteinase: Demonstration of Two Proteolytic Enzymes Produced by a Sarcina Strain (Coccus P)

Description: A Sarcina strain (Coccus P) produces two proteolytic enzymes. One is found only extracellularly, is far more prevalent, and is actively excreted during exponential growth. It is the enzyme responsible for the known strong proteolytic activity of the cultures of this strain. A second protease is, however, produced which remains associated with the intact cells but is released by the protoplasts. The two enzymes appear unrelated in their derivation. Calcium ions play an essential role in preventi… more
Date: June 29, 1970
Creator: Sarner, Nitza Z.; Bissel, Mina J.; Girolamo, Mario Di & Gorini, Luigi
Item Type: Refine your search to only Article
open access

Mechanism of Excretion of a Bacterial Proteinase: Factors Controlling Accumulation of the Extracellular Proteinase of a Sarcina Strain (Coccus P)

Description: It has been known that the extracellular proteinase of Coccus P is found only in cultures grown in the presence of Ca{sup 2+}. It is now shown that this cation is required neither for synthesis, excretion, or activation of a zymogen nor as a prosthetic factor necessary for enzymatic activity. The only function of Ca{sup 2+} is to stabilize the active structure of the enzyme molecule, presumably by substituting for absence of S-S bridges. In the absence of Ca{sup 2+} , the excreted proteinase un… more
Date: June 29, 1970
Creator: Bissell, Mina J.; Tosi, Roberto & Gorini, Luigi
Item Type: Refine your search to only Article
open access

Process control guidelines for CY 70 thorium campaign

Description: The report comprises five parts, with part I being an introduction. Part II consists of a general treatment of process control methods. Parts III through V discuss, in the flowsheet sequence, those problems pertinent to each equipment piece or system and provide operating guidelines. Specific operations that are somewhat different from those normally encountered in Purex are discussed at length. Operations routine to Purex can be found in the pertinent standard operating procedures. Part VI des… more
Date: July 29, 1970
Creator: Jackson, R. R.
Item Type: Refine your search to only Report
open access

Survey of Piledriver results and preliminary interpretation of three postshot cores in and near the cavity

Description: A summary of measured data collected by numerous agencies cooperating in the Piledriver event, is presented together with calculations based on empirical relations deduced from past events in comparable media. Of 9 relations that have been proposed to predict cavity radius, 5 result in radii accurate to <3.5%. The remainder are off by about 10 to 18%. Three postshot recovered cores (U-15.01 PS-1, -2, -3) were examined on a gross scale prior to extensive detailed sampling for laboratory tests… more
Date: April 29, 1970
Creator: Borg, I Y
Item Type: Refine your search to only Report
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